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Name: Biotin

Synonyms: d- Biotin; Vitamin H

Molecular Formula: C10H16N2O3

Molecular Weight: 244.31

CAS Number: 58-85-5

EINECS: 200-399-3

Character: Biotin is colorless to almost colorless crystalline Powder. Melting point 232 -233℃. Solubility in 25 (mg/100ml):water -22; 95% ethanol -80. Sparingly soluble in acidic or alkaline solution, can not soluble in other conventional organic solvent. Decompose when meet alkali or oxidant.

Specification: USP34

Item

Specification

Identification

To match with the reference IR spectrum

Specific rotation

+89° ~ +93°

Residual solvents (Toluene)

890ppm Max.

Assay

97.5 ~ 100.5%

Lead

2mg/kg Max.

Arsenic

1mg/kg Max.

Cadmium

1mg/kg Max.

Mercury

0.1mg/kg Max.

Mesh size

100% pass 300 mesh

Specification: EP7

Item

Specification

Characters

A white or almost white, crystalline powder

Identification

A.    IR: To match with the reference IR spectrum

B.     TLC: The principal spot in the chromatogram obtained with test solution is similar in position and size to the principal spot in the chromatogram obtained with reference solution

C.    Color reaction: The bromine water is decolorized

Appearance of solution

Clear and colorless

Specific optical rotation

+89° ~ +93°

Related substances

Meets EP requirement

Heavy metals

10ppm Max.

Loss on drying

1.0% Max.

Sulfated ash

0.1% Max.

Assay

98.5 ~ 101.0%

Mesh size

100% pass 300 mesh

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